HOLVES丨Microcarriers in Cell Culture: Your Questions Answered

Microcarrier (Microcarrier) means a class of microspherical scaffolds with a diameter of 50-350 μm and a density slightly higher than that of water, which have biological functions such as cell culture, proliferation and delivery. During the process of cell value-added differentiation, it can adhere to cells and provide a support matrix for cells.

Q1: What are microcarriers and what purpose do they serve in cell culture?

A1: Microcarriers are small, inert particles that provide a surface for anchorage-dependent cells to grow in suspension culture. They are typically made from materials such as polystyrene or gelatin, and their primary purpose is to increase the available surface area for cell attachment and proliferation. This technology is especially beneficial for scaling up the production of cells for various applications, including vaccine production and regenerative medicine.

Q2: How do microcarriers enhance cell growth compared to traditional culture methods?

A2: Microcarriers enhance cell growth by providing a three-dimensional environment that mimics natural tissue structures. Unlike traditional two-dimensional culture methods, microcarriers allow cells to grow in a more physiologically relevant manner, which can lead to improved cell viability and productivity. The increased surface area provided by microcarriers also allows for higher cell densities, making them ideal for large-scale bioproduction.

Q3: What types of cells can be cultured using microcarriers?

A3: A wide variety of cell types can be cultured using microcarriers, including adherent mammalian cells (such as CHO and HEK293 cells), stem cells, and some types of insect and plant cells. The choice of microcarrier type can depend on the specific requirements of the cell line being cultured, including nutrient needs, growth characteristics, and desired outcomes for the culture.

Q4: What are some key factors to consider when selecting microcarriers for cell culture?

A4: When selecting microcarriers for cell culture, several factors should be considered:

Material Composition: The material should be biocompatible and suitable for the specific cell type.

Size and Surface Properties: Microcarrier size affects the settling rate and cell attachment efficiency. Surface modifications can enhance cell growth and productivity.

Culture Conditions: Compatibility with the growth medium and ease of sterilization are also important.

Scalability: Consider whether the microcarrier system can be easily scaled up for large-scale production.

Q5: How are microcarriers used in bioprocessing applications?

A5: In bioprocessing applications, microcarriers are utilized for the mass production of cells and biological products. For instance, they are commonly employed in the production of vaccines, monoclonal antibodies, and gene therapies. By facilitating high-density cell culture in bioreactors, microcarriers help optimize yield and efficiency, enabling the rapid production of therapeutic proteins and cells for clinical use.

HOLVES develops and produces a wide range of cell culture carriers, including not only dextran-based microcarriers (HCD-001 and HCD-001G), but also biodegradable soluble 3D carriers (HCD-003) and polyester fiber carriers (HCF-001 and HSD-001) to meet the user's individual needs in the cell culture and purification process, and to facilitate the innovative development of the biopharmaceutical industry.

Laboratory Fermenter

Cla210 Laboratory Fermentor

Twin220 Parallel Fermenter

Hub240 Parallel Fermenter

Stainless Steel Fermenter

Su310 Stainless Steel Fermenter

Parallel Bioreactor

Photobioreactor

Bioreactor

Eu210 Fermenter/Bioreactor

Omini Cell510 Stainless Steel Bioreactor

Microcarriers

TFF System

Ceramic Membrane System

Bio Intelligent Pump

Microcarriers in Cell Culture: Your Questions Answered

Microcarrier (Microcarrier) means a class of microspherical scaffolds with a diameter of 50-350 m and a density slightly higher than that of water, which have biological functions such as cell culture, proliferation and delivery. During the process of cel

Microcarriers in Cell Culture: Your Questions Answered

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