About Microcarrier

• Microcarrier (Microcarrier) means a class of microspherical scaffolds with a diameter of 50-350 μm and a density slightly higher than that of water, which have biological functions such as cell culture, proliferation and delivery. During the process of cell value-added differentiation, it can adhere to cells and provide a support matrix for cells.

• HOLVES develops and produces a wide range of cell culture carriers, including not only dextran-based microcarriers (HCD-001 and HCD-001G), but also biodegradable gelatin 3D carriers (HCD-003 and HSD-003) and polyester fiber carriers (HCF-001 and HSD-001) to meet the user's individual needs in the cell culture and purification process, and to facilitate the innovative development of the biopharmaceutical industry.

HCD-001 microcarrier is a high-performance microcarrier made from dextran microspheres coated with animal-free recombinant collagen, significantly enhancing cell attachment efficiency and proliferation rate. It is particularly suitable for large-scale expansion of hard-to-culture cells (such as MSCs, Vero, MRC-5, 2BS, etc.) under serum-free culture conditions and is fully compatible with existing microcarrier-based cultivation processes.

The HCD-001G microcarrier undergoes gamma irradiation sterilization and is packaged in PC containers equipped with specialized transfer conduits to ensure sterility, meeting the stringent requirements of high-standard biological laboratories.

• Gentle and Efficient Harvesting: The easily hydrolysable surface recombinant collagen protects cell viability and ensures membrane integrity.
• Full System Compatibility: Compatible with various bioreactor systems, including stirred-tank, single-use reactors, and shake flasks, providing ample surface area for cell growth.
• High-Density Cultivation: Ultra-large surface area maximizes cell density.
• Flexible Process Support: Efficient separation technology allows easy isolation of cultures and cells from the medium, facilitating product collection. Perfectly suited for perfusion and continuous fed-batch cultures.
• Seamless Scale-Up Production: Supports scale-up processes for cell culture, including digestion-transfer or direct expansion strategies.

HCD-003 Gelatin 3D Microcarrier is made from medical-grade gelatin through an emulsion-porogen-crosslinking process. Its dry particle size ranges from 150–300 μm, with internal macropores of 20–50 μm (specific surface area ≥ 9000 cm²/g), providing a stable microenvironment for cell growth. Its natural biocompatibility supports high-density cultivation of conventional cells and significantly enhances the efficiency of hard-to-attach cells, making it a versatile solution in cell culture.

Due to the natural biocompatibility of gelatin, H3C-001 microcarriers are not only suitable for high-density culture of conventional cells, but also for cell types that are difficult to grow against the wall, making them a versatile and efficient choice for cell culture.

• 3D Structural Advantage: Unique 3D structure supports serum-free or low-serum culture of hard-to-attach cells (e.g., MSCs, MRC-5, Vero).
• High-Density Cultivation: Achieves over twice the density of MSCs compared to traditional dextran microcarriers.
• Efficient Recovery: Medical-grade gelatin material enables >90% enzymatic digestion recovery (compatible with trypsin and recombinant collagenase).
• Broad Application Scope: Suitable for cell therapy, exosome production, biopharmaceuticals, vaccine manufacturing, and cultivated meat industrialization.

Composed of medical-grade gelatin via precision engineering, it features macropores of 10–100 μm and rapidly disperses into independent flakes (>5 mm) upon hydration. This carrier is especially suitable for serum-free culture of hard-to-attach cells (e.g., MSCs, Vero) and low-serum high-density culture of 2BS cells, demonstrating excellent performance in fixed-bed and WAVE bioreactors. Its enzymatically degradable nature (compatible with trypsin/collagenase) ensures >90% cell recovery rate, significantly improving cell utilization efficiency. It is an ideal carrier for cell expansion and product expression.

• Large Flake Structure: Diamond/rectangular flakes (>5 mm) enable rapid dispersion and compatibility with fixed-bed reactors for scaled cultivation.
• Ultra-High Culture Density: Porous structure (10–100 μm, 9000 cm²/g specific surface area) supports over twice the MSC density of traditional carriers.
• Efficient Cell Recovery: Fully degradable by trypsin/collagenase with >90% recovery rate.
• Full System Compatibility: Applicable to various bioreactors including fixed-bed and WAVE systems, supporting diverse scenarios.
• Strong Cell Affinity: Naturally suitable for high-density culture of challenging adherent cells such as MSCs, MRC-5, and 2BS.
• Cross-Industry Applications: Ideal for cell therapy, exosome production, biopharmaceuticals, vaccine development, and cultivated meat production.