How to maintain, clean and repair the fermenters?[Part 1]

In order to ensure the normal operation of the fermenter, the user must not only be familiar with the working principle of the equipment and the structure of each component, performance role and the role of pipeline, valves, but also should pay attention to the maintenance of equipment and servicing. Only to achieve careful maintenance of equipment to ensure that the equipment is in the best condition and extend the working life of the equipment.


The maintenance and servicing of fermenter system can be divided into two ways, routine and regular. Neither can do without the other.

Routine maintenance (the monitoring content of the equipment in operation should be recorded accordingly)
  1. Whether the system pressure and vessel pressure are stable in the specified range.
  2. Whether the stirring system, temperature control system, sound of solenoid valve is normal.
  3. Whether the color of culture fluid is normal.
  4. Whether the temperature and dissolved oxygen pH display parameters are in line with the settings.
  5. Whether the liquid level in the vessel is normal.
  6. Whether there is leakage in the valve pipe joints and each interface.

Regular maintenance
Every three mouth,the user need to make a comprehensive inspection and correction of the equipment. Disconnect all power, water and air sources. Check all sealing rings and sealing ends of the system carefully. If there is permanent deformation, aging, scratches, damage, must be replaced. Check the sterilization filter intact, broken, blockage phenomenon should be replaced. Motor carbon brush wear and rectifier, bearing, coupler, mixing system condition.
Check all switches, buttons, electrical appliances, electronic components, fixing screws and nuts of electrical controllers for looseness and heat generation.

Cleaning and maintenance of fermenter vessel
  • Cleaning
The fermenter vessel body is the place where the material is in direct contact with. The degree of its surface finish and the cleanliness of internal components are the factors that directly affect the contamination of bacteria. Therefore, the vessel body and related equipment must be cleaned in time after each fermentation and before using again, and attention should be paid to the electrical components and electrode interface when cleaning, so that water and moisture cannot enter. Before cleaning, pH and DO electrodes should be removed and maintained according to their requirements.
Cleaning the vessel can be combined with water intake, motor agitation, heating together.If the water change for several times still can not clean, open the top cover with a soft brush to scrub the vessel parts.
The method is as follows: control the switch and power switch, remove the electrode, motor and its connecting plug. Unplug the air inlet hose condenser quick connection, remove the air inlet filter upper hose and connector. Remove the top cover together with the stirring vertically upwards and place it on a flat table, pad it well, do not collide with the shaft and impeller, and brush the vessel and internal parts with neutral detergent.
Note:The vessel cover can only grab the sleeve or top cover when moving and cleaning, not the mixing shaft, impeller and other easily deformed parts. At the same time of cleaning, the vessel and the export valve should be inspected for some O-ring seals, if permanent deformation, aging, scratches are found, they should be replaced in time. After cleaning and reinstalling, its appearance and vessel frame should be wiped dry with a rag. Tighten the six fastening screws of the lid, the force should be balanced and pay attention to the vessel and vessel seat gap balance. Glass fermenter vessel cleaning should be particularly careful, do not collide when disassembling; tighten the lid fastening screws, the force must be balanced, and pay attention to the gap balance, to avoid damage to the glass vessel body.
  • Leakage test
Install the electrode, motor, cable, inlet and outlet gas hose, condenser inlet and outlet water connector in place, and add air to the vessel for leakage test after installation. . Test run the system for 2~3 hours, if there is any problem, deal with it before it can be used officially.

Fermenter is not used for a long time
If the fermenter needs to be used again in the short term, it should be sterilized. Then pass in sterile air and keep pressure for use.
If you are ready to stop using it for a long time, you should sterilize it, then drain the water in the vessel , loosen the tightening screw of the vessel lid, take out the electrode and store it well, drain the remaining water from the vessel and the pipes, close all the valves and power supply, and put on the dust cover.
  • PS:
  1. The output pressure of the steam generator should be checked and adjusted before sterilizing.
  2. The air pressure in the vessel should be checked during the sterilization process.
  3. The output pressure of the air compressor should be checked and adjusted before fermenting.
  4. The air pressure in the vessel should be checked during the fermentation process.

Please look forward to the next content about the maintenance and servicing of pH electrode, DO electrode, stirring system, sterilization system, filter, etc.

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